With many thanks to Albym.See also
http://www.mccannpjfiles.co.uk/PJ/PORTU ... tm#p9p2307OUTROS APENSOS I - volume II pages 284-299
Comparative analysis report of biological vestiges collected from various locales 2008.03.31
GENETIC & BIOLOGICAL FORENSIC SERVICES
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Processo n* 2007/000226/LX-BC1
EXPERT REPORT
IDENTIFICATION OF BIOLOGICAL VESTIGES [remains] AND COMPARATIVE STUDY WITH REFERENCE SAMPLES
INFORMATION
REQUESTING ENTITY: Department of Criminal Investigation of Portimao of the Pollcia Judiciaria and National Directorate.
PROCESSO: NUIPC 201/07.0GALGS
NAME: MADELEINE BETH MCCANN.
OFFICIAL LETTER N.' 003610, dated 31/10/2007, that solicited the performance of lawful examinations of various samples of hair recovered from the following locations:
- Apartment C, 2' Floor, of Aparthotel Sol e Mar, in Burgau
- Vehicle type Audi, model A4, licence-plate 10-91-PF
- Vehicle type Renault, model Kangoo, licence-plate 07-20-UI
- Vehicle type Volkswagen, model Passat, licence-plate 57-12-HP
- Sofas located in in the garage of the CID Portimao
- Garden of apartment 5A, block A, tourist resort "OCEAN CLUB", Praia da Luz, Lagos.
OFFICIAL LETTER N.' 004080, dated 12/12/2007, that solicited "examinations as appropriate, with the view to determine the nature of the mark (found on the counterpane [quilt/bedspread] of one of the beds in apartment 5A of the tourist resort OCEAN CLUB in Praia da Luz) and if it permits the identification of the profile indicated by letter (L)".
OFFICIAL LETTER N.' 004079, dated 12/12/2007, that says "to send an item marked as fibre and a plastic item both extracted from the car boot..., material already examined..., soliciting the indulgence of the reader in the sense that further examinations are necessary on the aforementioned material, using a method considered adequate for the detection of possible biological remains that may not have been detected in the first examination to which they were subjected."
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OFFICIAL LETTER N.* 000550, dated de 04/07/07, that says to send 12 oral swabs [swatches] and hair collections, accompanied by respective letters of authorisation for their collection duly prepared and signed in the Departamento de Investigacao Criminal de Portimao da Policia Judiciaria in respect of the following people: Silvia Maria Correia Ramos Baptista (head-hair only), Mario Domingos Moreira, Luis Filipe Monteiro Ferro, Dina Maria dos Reis Rocha, Marina Paula Ramos Batista Castela, Maria Julia Serafim da Silva, Jose Maria Batista Roque, Jose Leal Pimentel, Antonio Conceicao Duarte, Nelson Filipe Pacheco da Costa, Maria Irene Trovao Ferro, Joao Francisco Pascoa Luis Trigo Barreiras e Vitor Manuel Martins.
OFFICIAL LETTER N.* 00551, dated 04/07/07, that says to send two oral swabs [swatches] and two capillary collections, obtaine from Michaela Walczuch e de Luis Filipe Duarte Antonio, accompanied by statements of swab collection and capillary collection duly prepared and signed in the Departamento de Investigacao Criminal de Portimao da Policia Judiciaria.
MATERIAL RECEIVED
In this 2nd fase, 187 hairs [generic], 2 pieces from the car boot and reference samples from 15 individuals were received.
In the 1st fase, were received 258 vestigios and 12 reference samples - oral swabs [swatches] from 11 individuals. The vestigios included 257 hairs [generic] and one speck detected in a cloth fragment collected from the counterpane of the bed next to the window of the bedroom of the children in apartament 5A (envelope 5), as mentioned in the previous report.
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DESCRIPTION OF THE MATERIAL RECEIVED
VESTIGIOS delivered by Policia Judiciaria on 08/11/2007:
With respect to Aparthotel Sol e Mar, 2', apartment C - Burgau
- 8 labelled envelopes with delivery note n' 420A/2007-CR/L:
Vestigio:
Vg1B- "Sundry head-hair collected from the kitchen floor of the apartment" - 20 hairs.
Vg2 - "Sundry head-hair collected at the entrance of the apartment" - 23 hairs.
Vg3 - "Sundry head-hair collected from the bedroom floor of the apartment" - 27 hairs.
Vg4 - "Sundry head-hair collected from the bed in the bedroom of the apartment" - 17 hairs.
Vg5 - "Sundry head-hair collected from the bathroom of apartment" - 16 hairs.
Vg6 - "Sundry head-hair collected from the floor of lounge of apartment" - 43 hairs.
Vg7 - "Sundry head-hair collected from a sofa presently in the lounge of the apartment"- 3 hairs.
Vg8 - "Sundry head-hair collected from a sofa presently in the lounge of the apartment"- 3 hairs.
With respect to Vehicle Audi A4 10-91-PF:
- 1 labelled envelope with delivery note n' 420C/2007-CR/L:
Vestigio:
Vg1 - "Sundry head-hair collected from the front seat, right side"- 3 hairs.
With respect to Vehicle Renault Kangoo 07-50-UI:
- 3 labelled envelopes with delivery note n' 420E/2007-CR/L:
Vestigio:
Vg1 - "Sundry head-hair collected from the row of seats at the rear of the Vehicle"- 1 hair.
Vg2 - "Sundry head-hair collected from the small area at the rear of Vehicle" - 2 hairs.
Vg3 - "Sundry head-hair collected from the front seat, right side" - 1 hair.
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With respect to Vehicle Volkswagen Passat 57-12-HP:
- 1 labelled envelope with delivery note n' 420F/2007-CR/L:
Vestigio:
Vg1 - "Sundry head-hair collected from the rear seat" - 6 hairs.
with respect to the sofas that are in the garage of the DIC Portimao:
- 3 labelled envelopes with delivery note n' 420B/2007-CR/L:
Vestigio:
Vg1 - "Sundry head-hair collected from the sofa having one seat"- 7 hairs.
Vg2 - "Sundry head-hair collected from the sofa having two seats"- 2 hairs.
Vg3 - "Sundry head-hair collected from the sofa having one seat" - 2 hairs.
With respect to the garden area of apartment 5A, tourist resort "OCEAN CLUB", Praia da Luz, Lagos:
- 1 labelled envelope with delivery note n' 420D/2007-CR/L:
Vestigio:
Vg25 - "Sundry head-hair" - 11 hairs.
VESTIGIOS delivered by Pol?ia Judiciaria on 14/12/2007:
With respect to the car boot:
- Pieces that included a fibre and plastic, having been previously analysed in various areas.
REFERENCE SAMPLES delivered by Pol?ia Judiciaria on 04/07/2007:
- 14 oral swabs [swatches] and collections of head-hair, relating to:
Mario Domingos Moreira, Luis Filipe Monteiro Ferro, Dina Maria dos Reis Rocha, Marina Paula Ramos Batista Castela, Maria Julia Serafim da Silva, Jose Maria Batista Roque, Jose Leal Pimentel, Antonio Conceicao Duarte, Nelson Filipe Pacheco da Costa, Maria Irene Trovao Ferro, Joao Francisco Pascoa Luis Trigo Barreiras, Vltor Manuel Martins, Michaela Walczuch e Luis Filipe Duarte Antonio.
- Collection of head-hair relatiing to:
Silvia Maria Correia Ramos Baptista.
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METHODOLOGY
1- Morphologic Analysis of the hairs
- Macro- and microscopic characterisation.
2- Nature of the sample
Phadebas Forensic test, for detection of saliva on the fragment of cloth corresponding to vestigio n'. 5 collected from the counterpane of the bed next to the window of the children's bedroom.
3- DNA Extraction:
DNA was extracted from the material received for analysis using one of the foloowing methods:
- Tissue and Hair Extraction kit-"DNA IQ(TM) System" (Promega)
- "QIAamp micro DNA kit" (Qiagen)
- Metodo de Chelex [1]
[1] Walsh P.S., Metzer D.A., Higuchi (1991). Chelex-100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10: 506-513.
4- Study of the nuclear DNA
After extraction the following genetic markers were studied using PCR:
- Autosomic STRs: D8S1179, D21Sl l, D7S820, CSFIPO, D3S1358, HUMTH01, D13S317, D16S539, D2S1338, D19S433, HUMVWA31/A, TPOX, D18S51, D5S818, HUMFIBRA/FGA and Amelogene, using primers and amplification conditions recommended in kit AmpFISTR(TM) Identifiler (Applied Bioystems).
- Autosomic STRs: D3S1358, HUMTH01, D21SII, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSFIPO, Penta D, HUMVWA31/A, D8S1179, TPOX, HUMFIBRA/FGA, and Amelogene using primers and amplification conditions recommended in kit PowerPlex(TM) 16 System (Promega).
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5 - Estudo do DNA mitocondrial
The mitochondrial DNA study (HVRI e HVRII) was performed in accordance with the following metodology:
PCR Amplification:
PCR amplification of the HVI e HVII regions of the human mtDNA, using specified primers amplification conditions in accordance with Vigilant et al., 1989 [2], Wilson et al., 1995 [3] e Alvarez-lglesias et al., [4].
[2] Vigilant L, Pennington R. Harpending H. Kocher TD, Wilson AC. ]989. "Mitochondrial DNA Sequences in Single Hairs from a Southern African Population". Proc Natl Acad Sci, USA, 1989, Vol. 86: 9350 - 9354.
[3] Wilson MR, DiZinno JA, Polanskey D, Replogle J. Budowle B. 1995. "Validation of Mitochondrial DNA Sequencing for Forensic Casework Analysis". Int J Legal Med. 1995, 108: 68 - 74.
[4] Alvarez-lglesias V, Jaime JC, Carracedo A, Salas A.2007. "Coding region mitochondrial DNA SNPs:Targeting East Asian and Native American haplogroups" Forensic Sci. Int. Genetics, 2007, 1 :44-55.
Sequenciation: Direct sequenciation of the HVI e HVII segments by capillary electrophoresis in the automatic sequenciator "ABI PRISM 3100 ou 3130 Genetic Analyser" (Applied Biosystems) with detection by fluorescence, using one of the kits:
- "BigDye(R) Terminator vl.1 Cycle Sequencing" (Applied Biosystems)
- "BigDye(R) Terminator v1.3 Cycle Sequencing" (Applied Biosystems)
- "dRhodamine Terminator Cycle Sequencing Ready Reaction" (Applied Biosystems)
The program "SeqScape(R) Software Version 2.0" (Applied Biosystems) was used to compare the sequences obtained from the analysed samples with the CRS (Cambridge Reference Sequence).
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RESULTS
[b]1- Morphologic analysis of the hairs
All the hairs received for analysis were subjected to macro- and microscopic characterisation. It was observed that some hairs were only stems while other had both root and stem. The majority of those with roots were found to be telogenic.
2- Nature of the sample
Phadebas Forensic test, for detection of saliva on the fragment of cloth corresponding to vestigio n'. 5 collected from the counterpane of the bed next to the window of the children's bedroom: Positive.
3- Study of the nuclear DNA
Autosomic STRs results were obtained from the following vestigios: root of one of the hairs collected from the bedroom floor of the Burgau apartment (Vg3) and in the lower zone of te fibre from the car boot. Table 1 includes, also, the profile of the spot from the counterpane, already sent previously.
Table 1 - Autosomic STRs of vestigios.
Code:
From the bedroom Vestigios inferior Spot on the cloth
SYSTEMS floor- Apart. in zone of the fibre fragment from the
Burgau -Vg 3 from the car boot counterpane
Amelogenina XY XY XY
D8S1179 13-15 10-11 12-13
D21S11 28-30 29 29-32.2
D7S820 9-10 10-12 10-12
CSFIPO 10-11 10-13 10-11
D3S1358 15-18 14-16 15-18
HUMTH01 6-9.3 9-9.3 9.3
D13S317 11 11-12 12-14
D16S539 11-12 10-14 11
D2S1338 17 18-24 18-25
D19S433 15 13-14 13-15
HUMVWA31/A 18-19 17-18 18
TPOX 8-11 8 11
D18S51 16-18 13-14 17-18
D5S818 12 11-13 12-13
HUMFIBRA/FGA 20 21-25 20-24
Penta E 7-8
Penta I> 9
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Translation:
GENETIC AND BIOLOGICAL FORENSIC SERVICES
Processo n' 2007/0005651PT-B
Processo n' 2007/000244/CR-B5,B6
Processo n' 2007/000226/LX-BCI
Autosomic STRs were also obtained relating to reference samples from:
Mario Domingos Moreira, Luis Filipe Monteiro Ferro, Dina Maria dos Reis Rocha, Marina Paula Ramos Batista Castela, Maria Julia Serafim da Silva, Jose Maria Batista Roque, Jose Leal Pimentel, Antonio Conceicao Duarte, Nelson Filipe Pacheco da Costa, Maria Irene Trovao Ferro, Joao Francisco Pascoa Luis Trigo Barreiras, Vitor Manuel Martins, Michaela Walczuch, Luis Filipe Duarte Antonio e Silvia Maria Correia Ramos Baptista.
4- Study of mitochondrial DNA
The vestigios delivered on 08/11/2007 and on 14/12/2007:
Mitochondrial DNA results were obtained in 137 hairs (roots and/or stems) from the 187 analysed. It was not possible to obtain any results from 46 hairs, and in 4 cases incomplete sequences were obtained.
5- Total of the samples studied:
Since May 2007, the following samples were analysed in the GENETIC AND BIOLOGICAL FORENSIC SERVICES offices of the North, Central e South Delegations of the Instituto Nacional of Medicina Legal:
- 25 reference samples.
- 447 vestigios, comprising:
>> 444 hairs, of which 432 were human;
>> 1 mark on a counterpane;
>> 2 objects from a car boot.
Of the 432 human hairs (roots and/or stems), 315 provided the results reflected in Table 2.
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Tabela 2. Haplotypes of Mitochondrial DNA of the vestigios and comparison with reference samples
Code:
Haplotypes No. of Collection point of vestigios Comparison with
samples reference samples
B 4 3 Entrance hall Ocean(Vg7),
1 Entrance hall Burgau(Vg2)
B1 1 1 Entrance hall Ocean(Vg7) Haplotype of Irene Ferro (IF)
13 Lounge floor Ocean(Vg6)
14 Entrance hall Ocean(Vg7)
C 42 3 Bedroom entrance Ocean(Vg1) Haplotype of Kate Healy (KH) and
1 Child's bed Ocean(Vg3) Haplotype of Jose Roque (JMBR)
4 Floor next to bed next window Ocean(Vg4)
7 Floor next to child's bed Ocean(Vg2)
C* 11 1 Entrance hall Ocean(Vg7)
10 Bedroom entrance Ocean(Vg1)
D 1 1 Lounge floor Ocean(Vg6)
D1 2 1 Bedroom entrance Ocean(Vg1)
1 Floor next to bed next window Ocean(Vg4)
E 1 1 Entrance hall Ocean(Vg7)
Ea 1 1 Sofa 1-seat; CID garage(Vg1)
F 3 1 Lounge floor Ocean(Vg6) and Haplotype of Vitor Martins (VM)
2 Entrance hall Ocean(Vg7) Haplotype of Vitor Martins (VM)
G 1 1 Lounge floor Ocean(Vg6) Haplotype of Matthew Oldfield (MO)
I 1 1 Entrance hall Ocean(Vg7) Haplotype of David Payne (DP)
1 Floor next to bed next window Ocean(Vg4)
J 3 1 Entrance hall Ocean(Vg7)
1 Floor next to child's bed Ocean(Vg2)
J1 0 Haplotype of Ant6nio Duarte(ACD)
K 16 1 Residencia Liliana(Vg1)
13 vehicle Volkswagen Envl(3), Env3(5), Haplotype of Michaela Walczuch (MW)
Env4(2), Env5(1), Env6(2) and of Luis Ferro (LFMF)
1 Renault(Vg3) e 1 Passat(Vg1)
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Tabela 2. Haplotypes of DNA Mitocondrial dos vestigios e comparacao com as samples of referencia enviadas (cont.)
Code:
Haplotypes No. of Collection point of vestigios Comparison with
samples reference samples
L 4 1 Mark counterpane bed (saliva)(env5)
3 Floor next to bed next window Ocean(Vg4)
La 4 1 Bedroom entrance Ocean(Vgl)
2 Entrance hall Ocean(Vg7)
1 Floor next to bed next window Ocean(Vg4)
L2 1 1 Vehicle Volkswagen (Env5)
M 27 13 Vehicle Volkswagen Envl(8), Env2(1), Env3(2), Env4(2)
13 Residencia Liliana(Vgl)
1 Bathroom Burgau(Vg5) Haplotype of Robert Murat (RQMU)
M* 22 22 Residencia Liliana(Vgl)
2 Lounge floor Ocean(Vg6)
10 Entrance hall Ocean(Vg7)
N 20 5 Bedroom entrance Ocean(Vgl) Haplotype of Gerald McCann (GM)
1 Floor next to bed next window Ocean(Vg4)
2 Floor next to child's bed Ocean(Vg2)
N* 4 1 Bedroom entrance Ocean(Vgl), 2 junto janela Ocean(Vg4)
1 Corridor Ocean(Vg7)
1 Lounge floor Ocean(Vg6),1 entrance hall
O 2 Ocean(Vg7) Haplotype of Russell O'Brien(RJB)
P 2 2 Garden Ocean(Vg5)
Q 2 1 Entrance hall Ocean(Vg7), 1 lounge floor Ocean(Vg6)
R 0 Haplotype of Nelson Costa (NFPC)
R* 1 1 Vehicle Volkswagen Env4
S 2 1 Bathroom (Vg5) Burgau and 1 Sofa (Vg7) Burgau Haplotype of Jane Tanner (JT)
T 8 1 LoungeFloor(Vg6), 2 Kitchen(Vgl),
2 EntHall(Vg2) Burgau, 3 BedFloor(Vg3) Burgau
Ta 2 1 Fl Kit(Vgl) and 1 Bedroom Floor(Vg3) Burgau
U 2 1 Entrance hall(Vg2) Burgau and 1 bed(Vg4) Burgau
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Tabela 2. Haplotypes of DNA Mitocondrial dos vestigios e comparacao com as samples of referencia enviadas (cont.)
Code:
Haplotypes No. of Collection point of vestigios Comparison with
samples reference samples
V 1 1 Lounge floor Ocean(Vg6)
V* 1 1 Renault(Vg2)
X 2 1 Bedroom entrance Ocean(Vgl) and 1 sofa 1-seat; garage(Vgl)
Y 2 1 sofa 1-seat; garage(Vgl) and 1 corridor Ocean(Vg7)
Z 3 l Residencia Liliana(Vgl)
2 sofa 1-seat garagem(Vgl e Vg3)
17 Ent.Hall(Vg2), 17 Kit.Floor(Vgl), 20 BedroomFloor(Vg3)
AA 85 Brg
13 LoungeFl(Vg6), 4 bathroom(Vg5),10 Bed&bedroom(Vg4)
Burgau
4 Sofa(Vg7 e 8) Burgau
AAa 6 3 Bed&bedroom(Vg4) and 3 bathroom(Vg5) Burgau
AA* 9 7 LoungeFloor(Vg6) Burgau
2 Vehicle Passat(Vgl)
AB 2 2 Audi A4(Vgl)
15 Haplotypes different from each other and from those above:
Code:
1 Bedroom entrance Ocean(Vgl) .
1 Bed, main bedroom Burgau(Vg4)
1 Sofa of 1-seat; garage(Vgl)
1 Floor next to bed next to window Ocean(Vg4)
1 Sofa of 2-seats; garage(Vg2)
1 Entrance hall Burgau(Vg2)
1 Bathroom Burgau(Vg5)
1 Sofa of 2-seats; garage(Vg2)
1 Bedroom floor Burgau(Vg3)
1 Entrance hall Ocean(Vg7)
1 Lounge floor Ocean(Vg6)
1 Garden Ocean(Vg25)
1 Vehicle Volkswagen (Env5)
1 Bathroom Burgau(Vg5)
1 Entrance hall Ocean(Vg7)
a Difference in 309.1C with respect to the corresponding Haplotype * Results obtained solely in HVI
1 One difference in regio HVII with repsect to the corresponsing Haplotype
2 Two differences in regio HVII with repsect to the corresponsing Haplotype
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CONCLUSIONS
First: In total in the two phases of the case there were 25 reference samples and 447 vestiges studied: 1 spot on the counterpane; 2 objects from the car boot and 444 hairs. The macro- and microscopic analyses of the 444 hairs revealed that 432 were humano and 12 non-human, no result being obtained for 98 hairs and only partial results for l9 hairs. With respect to the plastic object from the car boot no results were obtained.
Second: The search for saliva in the mark on the counterpane from one of the beds in apartament 5A of tourist resort Ocean Club was positive.
Third: The study of autosomic STRs from the sample received in the 2 phases revealed:
There were matches between the reference sample genetic profiles and the following analysed samples:
- hair root (CEnv 7-51) collected in apartament Ocean Club (entrance hall env.7) and Gerald Mccann, father of the victim.
- hair root (PVT07) collected in the Volkswagen 44-77-KD (floor in front of rear seats env.4) and Robert James Queriol Eveleight Murat.
- hair root (C742-05) collected in Volkswagen 44-77-KD (floor in front of front seats - env.3) and Michaela Walczuch.
There were no matches between the reference sample genetic profiles and the following analysed samples:
- Mark on the cloth fragment collected from apartament Ocean Club (counterpane of the bed next to the window of the children's bedroom - env. 5).
- Hair root collected in the apartament in Burgau (bedroom floor - vg3).
- Vestigios in the lower zone of the fibre from the car boot.
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Fourth: The mitochondrial DNA study revealed (a):
In the analysed samples from apartament 5-A of the Ocean Club:
- The Haplotype identified by the letter B1, present in one sample, and identical to that of Maria Irene Trovao Ferro (IF), meaning that sample was from her or an individual of the same maternal bloodline.
- The Haplotype identified by the letters C e C*, present in 53 samples, and identical to that of Kate Healy (KH), mother of the victim and to that of Jose Maria Batista Roque (JMBR), meaning those sample were from those persons or individuals of the same maternal bloodlines.
- The Haplotype identified by the letter F, present in 3 samples, and identical to that of Vltor Manuel Martins (VM), meaning those samples were from that person or individuals of the same maternal bloodlines.
- The Haplotype identified by the letter G, present in one sample, and identical to that of Matthew David Oldfield (MO), meaning that sample was from that person or an individual of the same maternal bloodline.
- The Haplotype identified by the letter I, present in one sample, and identical to that of David Anthony Payne (DP), meaning that sample was from that person or an individual of the same maternal bloodline.
- The Haplotype identified by the letters N e N*, present in 24 samples, and identical to that of Gerald Mccann (GM), father of the victim, meaning those sample were from that person or individuals of the same maternal bloodlines.
- The Haplotype identified by the letter O. present in 2 samples, and identical to that of Russell James O'Brien (RJB), meaning those samples were from that person or individuals of the same maternal bloodlines.
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In the analysed samples from other locations:
- The Haplotype identified by the letter K, present in 16 samples, (1 in the Residencia Liliana, 13 in vehicle Volkswagen, 1 in the Renault and l in the Passat) and identical to that of Michaela Walczuch (MW) and to that of Luis Filipe Monteiro Ferro (LFMF), meaning those samples were from those persons or individuals of the same maternal bloodlines.
- The Haplotype identified by the letters M e M*, present in 49 samples, (35 in the Residencia Liliana, 13 in the vehicle Volkswagen and 1 in the bathroom of the apartament in Burgau), and identical to that of Robert James Queriol Eveleight Murat (RQMU), meaning those samples were from that person or individuals of the same maternal bloodline.
- The Haplotype identified by the letter R*, present in one sample from the vehicle Volkswagen, and identical in HVI, to that of Nelson Filipe Pacheco da Costa (NFPC) - letter R, meaning that sample was from that person or an individual of the same amternal bloodline.
- The Haplotype identified by the letter S, present in 2 samples, (apartment in Burgau), and identical to that of Jane Michelle Tanner (JT), meaning those samples were from that person or individuals of the same maternal bloodline.
5a- The study of the mitochondrial DNA revealed also (a):
- The Haplotypes identified by letters D, D1, J, L, La, P e Q are different from those above, having been found solely in samples from apartament 5-A Ocean Club, not corresponding to any of the reference sample Haplotypes.
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- The Haplotypes identified by the letters L2, T, Ta, U e AB are also different from those above, having been found in the apartament in Burgau or in one of the vehicles, not corresponding to any of the reference sample Haplotypes:
Haplotype L2 - Vehicle Volkswagen
Haplotypes T, Ta - apartament in Burgau
Haplotype U - apartament in Burgau
Haplotype AB - Vehicle Audi A4
- The Haplotypes identified by the letters B, E, Ea, V, V*, X, Y, Z, AA, AAa e AA* are different from those above and distinct from the reference samples, having the same Haplotype been found in distinct locations:
Haplotype B - apart. Ocean Club and apart. Burgau
Haplotypes E, Ea - apart. Ocean Club and sofa of 1-seat in the garage in Portimao
Haplotypes V, V* - apart. Ocean Club and vehicle Renault
Haplotype X - apart. Ocean Club e sofa of 1-seat in the garage in Portimao
Haplotype Y - apart. Ocean Club e sofa of 1-seat in the garage in Portimao
Haplotype Z - Residencia Liliana e sofa of 1-seat in the garage in Portimao
Haplotypes AA, AAa, AA* - apartment in Burgau and vehicle Passat
- The Haplotype identified by the letter J1 corresponds to Antonio Conceicao Duarte (ACD).
This Haplotype was not identified in any of the analysed vestigios.
____________________________
(a) The Haplotypes C*, M*, N*, R*, V* e AA* are identical in the region HVI to the corresponding Haplotype, there not having been obtained any results in region HVIT.
The Haplotypes Ea. La, Ta e AAa presented a difference in 309.1C in relation to that of the corresponding Haplotype.
The Haplotypes Bl, Dl, Jl e Ll presented a difference in the region HVII in relation to that of the corresponding Haplotype.
The Haplotype L2 presented two differences in the region HVII in relation to that of the corresponding Haplotype.
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- The remaining 15 analysed vestigios reflected 15 Haplotypes different from each other and distinct from all of the above(including the reference samples).
Instituto Nacional of Medicina Legal, 31 of Marco of 2008
[centre]Director da Delegacao do Centro e Vogal do Conselho Directivo
Prof. Doutor Francisco Corte Real
Directora of Service - Delegacao do Norte
Prof. Doutora Maria of Fatima Pinheiro
Directora of Service - Delegacao do Centro
Dra. Maria Joao Anjos Porto
Directora of Service - Delegacao do Sul
Dra. Rosa Maria Espinheira
