With many thanks to Albym

I will translate after I have posted the 16 pages. [E&OE]
Note for the purist: the freeware OCR that I pulled down today does not recognise accented characters (and I am not going to retype them all).
============================
Manual page ref: 284-299
PDF ref: 02 - volume II - apenso I - Pages 2-17
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SERVICOS DE GENETICA E BIOLOGIA FORENSE

Processo n° 2007/000565/PT-B
Processo n° 2007/000244/CR-B5,B6
Processo n° 2007/000226/LX-BC1

RELATORlO PERICIAL

IDENTIFICACAO DE VESTIGIOS BIOLOGICOS E ESTUDO COMPARATIVO COM AMOSTRAS DE REFERENCIA

INFORMACAO

ENTIDADE REQUISITANTE: Departamento de Investigacao Criminal de Portimao da Pollcia Judiciaria e Directoria Nacional.

PROCESSO: NUIPC 201/07.0GALGS
NOME: MADELEINE BETH MCCANN.

OFICIO N.° 003610, datado de 31/10/2007, que solicita a realizacao dos competentes exames a diversas amostras de cabelos recolhidas nos seguintes locals:

- Apartamento C, 2° Andar, do Aparthotel Sol e Mar, no Burgau
- Viatura de marca Audi, modelo A4 e matricula 10-91-PF
- Viatura de marca Renault, modelo Kangoo e matricula 07-20-UI
- Viatura de marca Volkswagen, modelo Passat e matricula 57-12-HP
- Sofas que se encontravam na garagem do DIC Portimao
- Quintal do apartamento 5A, bloco A, Empreendimento Turistico "OCEAN CLUB", sito na Praia da Luz, em Lagos.

OFICIO N.° 004080, datado de 12/12/2007, que solicita "os exames tidos por convenientes, tento em vista determinar a natureza da mancha (encontrada na colcha de uma das camas do apartamento 5A do empreendimento turistico OCEAN CLUB da Praia da Luz) e que permitiu a identificacao do perfil indicado pela letra (L)".

OFICIO N.° 004079, datado de 12/12/2007, que diz "remeter um componente revestido a fibra e um componente de plastico ambos extraidos da bagageira..., material ja examinado.... solicitando-se a colaboracao de V.Exa. no sentido de se proceder aos necessarios exames ao citado material, com a metodologia considerada adequada tendo em vista a deteccao de eventuais vestigios biologicos que possam nao ter sido detectados no primeiro exame a que aquelas pecas foram submetidas".

1

SERVICOS DE GENETICA E BIOLOGIA FORENSE

Processo n° 2007/000565/PT-B
Processo n° 2007/000244/CR-B5,B6
Processo n° 2007/000226/LX-BC1

OFICIO N.° 000550, datado de 04/07/07, que diz enviar 12 zaragatoas bucais e recolhas de cabelos, acompanhadas das respectivas declaracoes de autorizacao de colheita devidamente assinadas e elaboradas no Departamento de Investigacao Criminal de Portimao da Policia Judiciaria relativas as seguintes pessoas: Silvia Maria Correia Ramos Baptista (so cabelos), Mario Domingos Moreira, Luis Filipe Monteiro Ferro, Dina Maria dos Reis Rocha, Marina Paula Ramos Batista Castela, Maria Julia Serafim da Silva, Jose Maria Batista Roque, Jose Leal Pimentel, Antonio Conceicao Duarte, Nelson Filipe Pacheco da Costa, Maria Irene Trovao Ferro, Joao Francisco Pascoa Luis Trigo Barreiras e Vitor Manuel Martins.

OFICIO N.° 00551, datado de 04/07/07, que diz enviar duas zaragatoas bucais e duas recolhas capilares, efectuadas nas pessoas de Michaela Walczuch e de Luis Filipe Duarte Antonio, acompanhadas com as declaracoes de recolha de zaragatoa e recolha capilar devidamente assinadas e elaboradas no departamento de Investigacao Criminal de Portimao da Policia Judiciaria.

MATERIAL RECEBIDO

Nesta 2a fase, foram recebidos 187 pelos, 2 pecas de bagageira e amostras de referencia relativas a 15 individuos.

Na 1a fase, foram recebidos 258 vestigios e 12 amostras de referencia - zaragatoas bucais relativas a 11 individuos. Os vestigios incluiram 257 pelos e uma mancha detectada em fragmento de tecido recolhido da colcha da cama junto a janela do quarto das criancas do apartamento 5A (envelope 5), mencionados no relatorio anterior.

2

SERVICOS DE GENETICA E BIOLOGIA FORENSE

Processo n° 2007/000565/PT-B
Processo n° 2007/000244/CR-B5,B6
Processo n° 2007/000226/LX-BC1

DESCRICAO DE MATERIAL RECEBIDO

VESTIGIOS entregues pela Policia Judiciaria em 08/11/2007:

Relativamente ao Aparthotel Sol e Mar, 2°, Apartamento C - Burgau

- 8 envelopes rotulados com guia de entrega n° 420A/2007-CR/L:
Vestigio:
Vg1B-"Diversos cabelos recolhidos no chao da cozinha do apartamento" - 20 pelos.
Vg2 - "Diversos cabelos recolhidos no corredor de entrada no apartamento" - 23 pelos.
Vg3 - "Diversos cabelos recolhidos no chao do quarto do apartamento" - 27 pelos.
Vg4 - "Diversos cabelos recolhidos na cama do quarto do apartamento" - 17 pelos.
Vg5 - "Diversos cabelos recolhidos na casa de banho do apartamento" - 16 pelos.
Vg6 - "Diversos cabelos recolhidos no chao da sala do apartamento" - 43 pelos.
Vg7 - "Diversos cabelos recolhidos num sofa existente na sala do apartamento"- 3 pelos.
Vg8 - "Diversos cabelos recolhidos num sofa existente na sala do apartamento"- 3 pelos.

Relativamente a Viatura Audi A4 10-91-PF:

- 1 envelope rotulado com guia de entrega n° 420C/2007-CR/L:
Vestigio:
Vg1 - "Diversos cabelos recolhidos sobre o banco frontal do lado direito"- 3 pelos.

Relativamente a Viatura Renault Kangoo 07-50-UI:

- 3 envelopes rotulados com guia de entrega n° 420E/2007-CR/L:
Vestigio:
Vg1 - "Diversos cabelos recolhidos sobre a bancada artesanal existente na caixa fechada da viatura"- 1 pelo.
Vg2 - "Diversos cabelos recolhidos no habitaculo traseiro da viatura" - 2 pelos.
Vg3 - "Diversos cabelos recolhidos no banco frontal do lado direito" - I pelo.

3

SERVICOS DE GENETICA E BIOLOGIA FORENSE

Processo n° 2007/000565/PT-B
Processo n° 2007/000244/CR-B5,B6
Processo n° 2007/000226/LX-BC1

Relativamente a Viatura Volkswagen Passat 57-12-HP:

- 1 envelope rotulado com guia de entrega n° 420F/2007-CR/L:
Vestigio:
Vg1 - "Diversos cabelos recolhidos sobre o banco traseiro" - 6 pelos.

Relativamente aos sofas que se encontravam na garagem do DIC Portimao:

- 3 envelopes rotulados com guia de entrega n° 420B/2007-CR/L:
Vestigio:
Vg1 - "Diversos cabelos recolhidos num sofa de um lugar"- 7 pelos.
Vg2 - "Diversos cabelos recolhidos num sofa de dois lugares"- 2 pelos.
Vg3 - "Diversos cabelos recolhidos num sofa de um lugar" - 2 pelos.

Relativamente ao quintal do apartamento 5A, Empreendimento Turistico "OCEAN CLUB", Praia da Luz, Lagos:

- 1 envelope rotulado com guia de entrega n° 420D/2007-CR/L:
Vestigio:
Vg25 - "Diversos cabelos" - 11 pelos.

VESTIGIOS entregues pela Policia Judiciaria em 14/12/2007:

Relativamente a Bagageira:

- Pecas que incluem um componente revestido a fibra e um componente de plastico, tendo sido efectuada analise em varias zonas.

AMOSTRAS DE REFERENCIA entregues pela Policia Judiciaria em 04/07/2007:

- 14 Zaragatoas bucais e recolhas de cabelos, relativas a:

Mario Domingos Moreira, Luis Filipe Monteiro Ferro, Dina Maria dos Reis Rocha, Marina Paula Ramos Batista Castela, Maria Julia Serafim da Silva, Jose Maria Batista Roque, Jose Leal Pimentel, Antonio Conceicao Duarte, Nelson Filipe Pacheco da Costa, Maria Irene Trovao Ferro, Joao Francisco Pascoa Luis Trigo Barreiras, Vltor Manuel Martins, Michaela Walczuch e Luis Filipe Duarte Antonio.

- Recolha de cabelos, relativa a:

Silvia Maria Correia Ramos Baptista.

4

SERVICOS DE GENETICA E BIOLOGIA FORENSE

Processo n° 2007/000565/PT-B
Processo n° 2007/000244/CR-B5,B6
Processo n° 2007/000226/LX-BC1

METODOLOGIA

1- Analise morfologica dos pelos

- Caracteriza,cao macroscopica e microscopica.

2- Natureza da Amostra

Teste Phadebas Forensic, para deteccao de saliva efectuada em pequena mancha do fragmento de tecido correspondente ao vestlgio n° 5 recolhido da colcha da cama junto a janela do quarto das criancas.

3- Extraccao de DNA:

O DNA do material recebido para analise, foi extraido por um dos seguintes metodos

- Tissue and Hair Extraction kit-"DNA IQ(TM) System" (Promega)
- "QIAamp micro DNA kit" (Qiagen)
- Metodo de Chelex [1]

[1] Walsh P.S., Metzer D.A., Higuchi (1991). Chelex-100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10: 506-513.

4- Estudo do DNA nuclear

Apos a extraccao foram estudados os seguintes marcadores geneticos, por PCR:

- Os STRs autossomicos: D8S1179, D21Sl l, D7S820, CSFIPO, D3S1358, HUMTH01, D13S317, D16S539, D2S1338, D19S433, HUMVWA31/A, TPOX, D18S51, D5S818, HUMFIBRA/FGA e a amelogenina, utilizando os primers e as condicoes de amplificacao sugeridas no kit AmpFISTR(TM) Identifiler (Applied Bioystems).

- Os STRs autossomicos: D3S1358, HUMTH01, D21SII, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSFIPO, Penta D, HUMVWA31/A, D8S1179, TPOX, HUMFIBRA/FGA, e a amelogenina utilizando os primers e as condi~oes de amplificacao sugeridas no kit PowerPlex(TM) 16 System (Promega).

5

SERVICOS DE GENETICA E BIOLOGIA FORENSE

Processo n° 2007/000565/PT-B
Processo n° 2007/000244/CR-B5,B6
Processo n° 2007/000226/LX-BCI

5 - Estudo do DNA mitocondrial

O estudo do DNA mitocondrial (HVRI e HVRII) foi efectuado de acordo com a seguinte metodologia:

Amplificacao por PCR:
Amplificacao por PCR das regioes HVI e HVII do mtDNA humano, utilizando primers especificos e condicoes de amplificacao de acordo com Vigilant et
al., 1989 [2], Wilson et al., 1995 [3] e Alvarez-lglesias et al., [4].

[2] Vigilant L, Pennington R. Harpending H. Kocher TD, Wilson AC. ]989. "Mitochondrial DNA Sequences in Single Hairs from a Southern African Population". Proc Natl Acad Sci, USA, 1989, Vol. 86: 9350 - 9354.
[3] Wilson MR, DiZinno JA, Polanskey D, Replogle J. Budowle B. 1995. "Validation of Mitochondrial DNA Sequencing for Forensic Casework Analysis". Int J Legal Med. 1995, 108: 68 - 74.
[4] Alvarez-lglesias V, Jaime JC, Carracedo A, Salas A.2007. "Coding region mitochondrial DNA SNPs:Targeting East Asian and Native American haplogroups" Forensic Sci. Int. Genetics, 2007, 1 :44-55.

Sequenciacao: Sequencia,cao directa dos segmentos HVI e ~VII por electroforese capilar em sequenciador automatico "ABI PRISM 3100 ou 3130 Genetic Analyser" (Applied Biosystems) com deteccao por fluorescencia, usando um dos kits:

- "BigDye(R) Terminator vl.1 Cycle Sequencing" (Applied Biosystems)
- "BigDye(R) Terminator v1.3 Cycle Sequencing" (Applied Biosystems)
- "dRhodamine Terminator Cycle Sequencing Ready Reaction" (Applied Biosystems)

Foi utilizado o programa "SeqScape(R) Software Version 2.0" (Applied Biosystems), para comparacao das sequencias obtidas nas amostras analisadas, com a CRS (Cambridge Reference Sequence).

6

SERVIOS DE GENETICA E BIOLOGIA FORENSE

Processo n° 2007/000565/PT-B
Processo n° 2007/000244/CR-B5,B6
Processo n° 2007/000226/LX-BC1

RESULTADOS

[b]1- Analise morfologica dos pelos

Todos os pelos recebidos para analise foram sujeitos a uma caracteriza,cao macroscopica e microscopica. Observaram-se pelos so com haste e outros com haste e bolbo. A maioria dos que possu~am bolbo, encontravam-se em fase telogenica.

2- Natureza da Amostra

Teste Phadebas Forensic, para deteccao de saliva em fragmento de tecido recolhido da colcha da cama junto a janela do quarto das criancas: Positivo.

3- Estudo do DNA nuclear

Foram obtidos resultados de STRs autossomicos nos seguintes vestigios: bolbo de um dos pelos recolhidos no chao do quarto do apartamento do Burgau (Vg 3) e na zona inferior da peca em fibra da bagageira. A Tabela I inclui, tambem, o perfil da mancha da colcha, ja enviado anteriormente.

Tabela 1 - STRs autossomicos de vestigios.


7
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My TRANSLATION (subject to correction by a real PT-speaker, as always)

GENETIC & BIOLOGICAL FORENSIC SERVICES

Processo n° 2007/000565/PT-B
Processo n° 2007/000244/CR-B5,B6
Processo n° 2007/000226/LX-BC1

EXPERT REPORT

IDENTIFICATION OF BIOLOGICAL VESTIGES [remains] AND COMPARATIVE STUDY WITH REFERENCE SAMPLES

INFORMATION

REQUESTING ENTITY: Departament of Criminal Investigation of Portimao of the Pollcia Judiciaria and National Directorate.

PROCESSO: NUIPC 201/07.0GALGS
NAME: MADELEINE BETH MCCANN.

OFFICIAL LETTER N.° 003610, dated 31/10/2007, that solicited the performance of lawful examinations of various samples of hair recovered from the following locations:

- Apartament C, 2° Floor, of Aparthotel Sol e Mar, in Burgau
- Vehicle type Audi, model A4, licence-plate 10-91-PF
- Vehicle type Renault, model Kangoo, licence-plate 07-20-UI
- Vehicle type Volkswagen, model Passat, licence-plate 57-12-HP
- Sofas located in in the garage of the CID Portimao
- Garden of apartament 5A, block A, tourist resort "OCEAN CLUB", Praia da Luz, Lagos.

OFFICIAL LETTER N.° 004080, dated 12/12/2007, that solicited "examinations as approriate, with the view to determine the nature of the mark (found on the counterpane [quilt/bedspread] of one of the beds in apartament 5A of the tourist resort OCEAN CLUB in Praia da Luz) and if it permits the identification of the profile indicated by letter (L)".

OFFICIAL LETTER N.° 004079, dated 12/12/2007, that says "to send an item marked as fibre and a plastic item both extracted from the car boot..., material already examined..., soliciting the indulgence of the reader in the sense that further examinations are necessary on the aforementioned material, using a method considered adequate for the detection of possible biological remains that may not have been detected in the first examination to which they were subjected."

1

GENETIC & BIOLOGICAL FORENSIC SERVICES

Processo n° 2007/000565/PT-B
Processo n° 2007/000244/CR-B5,B6
Processo n° 2007/000226/LX-BC1

OFFICIAL LETTER N.° 000550, dated de 04/07/07, that says to send 12 oral swabs [swatches] and hair collections, accompanied by respective letters of authorisation for their collection duly prepared and signed in the Departamento de Investigacao Criminal de Portimao da Policia Judiciaria in respect of the following people: Silvia Maria Correia Ramos Baptista (head-hair only), Mario Domingos Moreira, Luis Filipe Monteiro Ferro, Dina Maria dos Reis Rocha, Marina Paula Ramos Batista Castela, Maria Julia Serafim da Silva, Jose Maria Batista Roque, Jose Leal Pimentel, Antonio Conceicao Duarte, Nelson Filipe Pacheco da Costa, Maria Irene Trovao Ferro, Joao Francisco Pascoa Luis Trigo Barreiras e Vitor Manuel Martins.

OFFICIAL LETTER N.° 00551, dated 04/07/07, that says to send two oral swabs [swatches] and two capillary collections, obtaine from Michaela Walczuch e de Luis Filipe Duarte Antonio, accompanied by statements of swab collection and capillary collection duly prepared and signed in the Departamento de Investigacao Criminal de Portimao da Policia Judiciaria.

MATERIAL RECEIVED

In this 2nd fase, 187 hairs [generic], 2 pieces from the car boot and reference samples from 15 individuals were received.

In the 1st fase, were received 258 vestigios and 12 reference samples - oral swabs [swatches] from 11 individuals. The vestigios included 257 hairs [generic] and one speck detected in a cloth fragment collected from the counterpane of the bed next to the window of the bedroom of the children in apartament 5A (envelope 5), as mentioned in the previous report.

2

GENETIC & BIOLOGICAL FORENSIC SERVICES

Processo n° 2007/000565/PT-B
Processo n° 2007/000244/CR-B5,B6
Processo n° 2007/000226/LX-BC1

DESCRIPTION OF THE MATERIAL RECEIVED

VESTIGIOS delivered by Policia Judiciaria on 08/11/2007:

With respect to Aparthotel Sol e Mar, 2°, apartment C - Burgau

- 8 labelled envelopes with delivery note n° 420A/2007-CR/L:
Vestigio:
Vg1B- "Sundry head-hair collected from the kitchen floor of the apartment" - 20 hairs.
Vg2 - "Sundry head-hair collected at the erntrance of the apartment" - 23 hairs.
Vg3 - "Sundry head-hair collected from the bedroom floor of the apartment" - 27 hairs.
Vg4 - "Sundry head-hair collected from the bed in the bedroom of the apartment" - 17 hairs.
Vg5 - "Sundry head-hair collected from the bathroom of apartment" - 16 hairs.
Vg6 - "Sundry head-hair collected from the floor of lounge of apartment" - 43 hairs.
Vg7 - "Sundry head-hair collected from a sofa presently in the lounge of the apartment"- 3 hairs.
Vg8 - "Sundry head-hair collected from a sofa presently in the lounge of the apartment"- 3 hairs.

With respect to Vehicle Audi A4 10-91-PF:

- 1 labelled envelope with delivery note n° 420C/2007-CR/L:
Vestigio:
Vg1 - "Sundry head-hair collected from the front seat, right side"- 3 hairs.

With respect to Vehicle Renault Kangoo 07-50-UI:

- 3 labelled envelopes with delivery note n° 420E/2007-CR/L:
Vestigio:
Vg1 - "Sundry head-hair collected from the row of seats at the rear of the Vehicle"- 1 hair.
Vg2 - "Sundry head-hair collected from the small area at the rear of Vehicle" - 2 hairs.
Vg3 - "Sundry head-hair collected from the front seat, right side" - 1 hair.

3

GENETIC & BIOLOGICAL FORENSIC SERVICES

Processo n° 2007/000565/PT-B
Processo n° 2007/000244/CR-B5,B6
Processo n° 2007/000226/LX-BC1

With respect to Vehicle Volkswagen Passat 57-12-HP:

- 1 labelled envelope with delivery note n° 420F/2007-CR/L:
Vestigio:
Vg1 - "Sundry head-hair collected from the rear seat" - 6 hairs.

with respect to the sofas that are in the garage of the DIC Portimao:

- 3 labelled envelopes with delivery note n° 420B/2007-CR/L:
Vestigio:
Vg1 - "Sundry head-hair collected from the sofa having one seat"- 7 hairs.
Vg2 - "Sundry head-hair collected from the sofa having two seats"- 2 hairs.
Vg3 - "Sundry head-hair collected from the sofa having one seat" - 2 hairs.

With respect to the garden area of apartment 5A, tourist resort "OCEAN CLUB", Praia da Luz, Lagos:

- 1 labelled envelope with delivery note n° 420D/2007-CR/L:
Vestigio:
Vg25 - "Sundry head-hair" - 11 hairs.

VESTIGIOS delivered by Policia Judiciaria on 14/12/2007:

With respect to the car boot:

- Pieces that included a fibre and plastic, having been previously analysed in various areas.

REFERENCE SAMPLES delivered by Policia Judiciaria on 04/07/2007:

- 14 oral swabs [swatches] and collections of head-hair, relating to:

Mario Domingos Moreira, Luis Filipe Monteiro Ferro, Dina Maria dos Reis Rocha, Marina Paula Ramos Batista Castela, Maria Julia Serafim da Silva, Jose Maria Batista Roque, Jose Leal Pimentel, Antonio Conceicao Duarte, Nelson Filipe Pacheco da Costa, Maria Irene Trovao Ferro, Joao Francisco Pascoa Luis Trigo Barreiras, Vltor Manuel Martins, Michaela Walczuch e Luis Filipe Duarte Antonio.

- Collection of head-hair relatiing to:

Silvia Maria Correia Ramos Baptista.

4

GENETIC & BIOLOGICAL FORENSIC SERVICES

Processo n° 2007/000565/PT-B
Processo n° 2007/000244/CR-B5,B6
Processo n° 2007/000226/LX-BC1

METHODOLOGY

1- Morphologic Analysis of the hairs

- Macro- and microscopic characterisation.

2- Nature of the sample

Phadebas Forensic test, for detection of saliva on the fragment of cloth corresponding to vestigio n°. 5 collected from the counterpane of the bed next to the window of the children's bedroom.

3- DNA Extraction:

DNA was extracted from the material received for analysis using one of the foloowing methods:

- Tissue and Hair Extraction kit-"DNA IQ(TM) System" (Promega)
- "QIAamp micro DNA kit" (Qiagen)
- Metodo de Chelex [1]

[1] Walsh P.S., Metzer D.A., Higuchi (1991). Chelex-100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10: 506-513.

4- Study of the nuclear DNA

After extraction the following genetic markers were studied using PCR:

- Autosomic STRs: D8S1179, D21Sl l, D7S820, CSFIPO, D3S1358, HUMTH01, D13S317, D16S539, D2S1338, D19S433, HUMVWA31/A, TPOX, D18S51, D5S818, HUMFIBRA/FGA and Amelogene, using primers and amplification conditions recommended in kit AmpFISTR(TM) Identifiler (Applied Bioystems).

- Autosomic STRs: D3S1358, HUMTH01, D21SII, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSFIPO, Penta D, HUMVWA31/A, D8S1179, TPOX, HUMFIBRA/FGA, and Amelogene using primers and amplification conditions recommended in kit PowerPlex(TM) 16 System (Promega).

5

GENETIC & BIOLOGICAL FORENSIC SERVICES

Processo n° 2007/000565/PT-B
Processo n° 2007/000244/CR-B5,B6
Processo n° 2007/000226/LX-BCI

5 - Estudo do DNA mitocondrial

The mitochondrial DNA study (HVRI e HVRII) was performed in accordance with the following metodology:

PCR Amplification:
PCR amplification of the HVI e HVII regions of the human mtDNA, using specified primers amplification conditions in accordance with Vigilant et al., 1989 [2], Wilson et al., 1995 [3] e Alvarez-lglesias et al., [4].

[2] Vigilant L, Pennington R. Harpending H. Kocher TD, Wilson AC. ]989. "Mitochondrial DNA Sequences in Single Hairs from a Southern African Population". Proc Natl Acad Sci, USA, 1989, Vol. 86: 9350 - 9354.
[3] Wilson MR, DiZinno JA, Polanskey D, Replogle J. Budowle B. 1995. "Validation of Mitochondrial DNA Sequencing for Forensic Casework Analysis". Int J Legal Med. 1995, 108: 68 - 74.
[4] Alvarez-lglesias V, Jaime JC, Carracedo A, Salas A.2007. "Coding region mitochondrial DNA SNPs:Targeting East Asian and Native American haplogroups" Forensic Sci. Int. Genetics, 2007, 1 :44-55.

Sequenciation: Direct sequenciation of the HVI e HVII segments by capillary electrophoresis in the automatic sequenciator "ABI PRISM 3100 ou 3130 Genetic Analyser" (Applied Biosystems) with detection by fluorescence, using one of the kits:

- "BigDye(R) Terminator vl.1 Cycle Sequencing" (Applied Biosystems)
- "BigDye(R) Terminator v1.3 Cycle Sequencing" (Applied Biosystems)
- "dRhodamine Terminator Cycle Sequencing Ready Reaction" (Applied Biosystems)

The program "SeqScape(R) Software Version 2.0" (Applied Biosystems) was used to compare the sequences obtained from the analysed samples with the CRS (Cambridge Reference Sequence).

6

SERVIOS DE GENETICA E BIOLOGIA FORENSE

Processo n° 2007/000565/PT-B
Processo n° 2007/000244/CR-B5,B6
Processo n° 2007/000226/LX-BC1

RESULTS

[b]1- Morphologic analysis of the hairs

All the hairs received for analysis were subjected to macro- and microscopic characterisation. It was observed that some hairs were only stems while other had both root and stem. The majority of those with roots were found to be telogenic.

2- Nature of the sample

Phadebas Forensic test, for detection of saliva on the fragment of cloth corresponding to vestigio n°. 5 collected from the counterpane of the bed next to the window of the children's bedroom: Positive.

3- Study of the nuclear DNA

Autosomic STRs results were obtained from the following vestigios: root of one of the hairs collected from the bedroom floor of the Burgau apartment (Vg3) and in the lower zone of te fibre from the car boot. Table 1 includes, also, the profile of the spot from the counterpane, already sent previously.

Table 1 - Autosomic STRs of vestigios.


7

FIRST INML REPORT 9 JULY 2007

NATIONAL INSTITUTE OF LEGAL MEDICINE
GENETIC AND BIOLOGICAL FORENSIC SERVICES

Processo n° 2007/000268/P-B
Processo n° 2007/000244/CR-B
Processo n° 2007/000226/LX-BC

EXPERT REPORT

IDENTIFICATION OF BIOLOGICAL TRACES AND COMPARATIVE STUDY WITH REFERENCE SAMPLES

INFORMATION

REQUESTING AGENCY: Departamento de Investigacao Criminal de Portimao da Policia Judiciaria e Directoria Nacional.

PROCESSO: NUIPC 201/07.0GALGS
NOME: MADELEINE BETH MCCANN.

OFICIO N.° 013.079 and fax n° 399, both dated 08/05/2007, that say "to proceed to deliver the traces recovered from apartment n° 5-A of the tourist resort "The Ocean Club" in Praia da Luz in Lagos", which were accompanied by 7 (seven) sealed envelopes under a delivery note n° 156/2007-CR/L, as part of the investigation into the disappearance of the child MADELEINE BETH MCCANN.

OFICIO N.° 13443 and fax n° 413 both dated 15/05/2007, that say to proceed to deliver the traces recovered from the residence at Rua do Ramalhete, Casa Liliana in Praia da Luz in Lagos (1 envelope), and from the Volkswagen with numberplate 44-77-KD (7 sealed envelopes), which were accompanied by delivery notes n° 170A/2007CR/L and 170B/2007-CR/L. respectively.

FAX N° 000415 dated 15/05/2007, that requests the INML to proceed with all necessary examinations on all the traces delivered to them as part of the abovementioned Inquiry.

OFICIO N.° 13453 /I /2007 dated 15/05/2007, that says to proceed to deliver the bucal swabs recovered from Matthew David Oldfield, Jane Michelle Tanner, Gerald Patrick Mccann, Kate Marie Healy, with delivery note n° 170C/2007-CR/L and relevant official documents from the Departamento de Investigacao Criminal de Portimao da Policia Judiciaria.

OFICIO N.° 001803 dated 17/05/07, that says "to remit bucal swabs recovered from individuals, asking for comparisons of DNA profiles with those from biological traces recovered from various locations, as part of the present legal documents", accompanied by relevant official documents from the Departamento de lnvestigacao Criminal de Portimao da Policia Judiciaria.

OFICIO N.° 002026 dated 05/06/07, that says "to deliver two bucal swabs provided by Robert James Queriol Eveleigh Murat", with relevant official documents from the Departamento de Investigacao Criminal de Portimao da Policia Judiciaria.



There were received 258 traces and 12 reference samples - bucal swabs from 11 individuals.

DESCRIPTION OF MATERIAL RECEIVED

TRACES
Relating to Apartment n° 5-A in the tourist resort "The Ocean Club" Praia da Luz-Lagos:

- 32 hairs in envelope n° 1 recovered from the floor at the entrance to the children's bedroom.

- 28 hairs in envelope n° 2 recovered from the floor next to the bed from which the child disappeared.

- 4 hair in envelope n° 3 recovered from the top of the bed from which the child disappeared.

- 15 hairs in envelope n° 4 recovered from the floor next to the bed that was next to the window in the children's bedroom.

- 1 piece of cloth in envelope n° 5 recovered from the bedspread of the bed next to the window in the children's bedroom.
Fragment of cloth, mauve/violet in colour with square motifs, circular in form about 10cm in diameter. A small fluorescent spot is observed under a Crime-light.

- 31 hairs in envelope N° 6 recovered from the floor of the lounge.

- 58 hairs in envelope N° 7 recovered from the entrance hall at the front door of the apartment.

[The above were] Delivered by the Policia Judiciaria on 08/05/2007.

Relating to the Volkswagen 44-77-KD:

- 15 hairs in envelope N° 1 recovered from the front-right seat.

- 2 hairs in envelope N° 2 recovered from the front-left seat.

- 12 hairs in N° 3 recovered from the floor in front of the front seats.

- 7 hairs in envelope N° 4 recovered from the floor in front of the rear seat.

- 4 hairs in envelope N° 5 recovered from the rear seat.

- 5 hairs in envelope N° 6 recovered from the rear part of the vehicle.

- 4 hairs in envelope N° 7 recovered from the internal covering of the roof.

Relating to Residencia Casa Liliana - Praia da Luz-Lagos:

- 40 hairs in envelope N° 1 recovered from the bed in the bedroom of of the suspect.

[The above were] Delivered by the Policia Judiciaria on 15/05/2007.

REFERENCE SAMPLES

- 4 sealed bucal swabs from:
Matthew David Oldfield, Jane Michelle Tanner, Gerald Patrick Mccann, Kate Marie Healey.

Delivered by the Policia Judiciaria on 15/05/2007.

- 6 sealed bucal swabs from:
Dianne Webster, Rachael Mariamma Jean Mampilly, Russell James O'Brien, Fiona Payne, David Anthony Payne, Silvia Maria Correia Batista.

Delivered by the Policia Judiciaria on 18/05/2007.

- 2 sealed bucal swabs from:
Robert James Queriol Eveleigh Murat.

Remitted by the Departamento de Investigacao Criminal de Portimao da Policia Judiciaria and delivered on 06/06/2007.

METHODOLOGY

1-Morphology analysis of hairs

- Macro- and microscopic characterisation.

2-Nature of the sample

Acid Phosphatase Test to detect semen on the small spot on the cloth fragment in envelope n° 5 recovered from the bedspread of the bed next to the window of the children's bedroom.
[Ref: http://www.semenonpanties.com/laux.htm ]

3- DNA extraction:

The following methods were used to extract DNA from the material received for analysis:

- Extraction with phenol-chloroform (organic extraction) [1,2]
[Ref: http://en.wikipedia.org/wiki/Phenol-chl ... extraction ]
- Tissue and Hair Extraction kit -"DNA IQ(tm) System" (Promega)
- "QIAamp micro DNA kit" method (Qiagen)
- Chelex method [3]

[1] Huhne, J., Pfiffer, H., Waterkamp, K., Brinkmann, B. 1999. "Mitochondrial DNA in human hair shafts existence of intra-individual differences?". International Journal of Legal Medicine (1999), 112: 172-175.
[2] Maniatis T. Fritsch EF, Sambrook J. 1982. "Molecular Cloning - A Laboratory Manual". Cold Spring Harbor Laboratory.
[3] Walsh P.S., Metzer D.A., Higuchi (1991). Chelex-100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10: 506-513.

4-Nuclear DNA study

After extraction the following genetic markers were studied by PCR:

· Autosomic STRs: D8S1179, D21S11, D7S820, CSFlPO, D3S1358, HUMTH01, D13S317, D16S539, D2S1338, D19S433, HUMVWA31/A, TPOX, D18S51, D5S818, HUMFIBRA/FGA and Amelogene, using the primers and conditions of amplification suggested in the kit AmpFISTR Identifiler (Applied Bioystems).

· Autosomic STRs: D3S1358, HUMTH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSFlPO, Penta D, HUMVWA31/A, D8S1179, TPOX, HUMFIBRA/FGA, and Amelogene using the primers and the conditions of amplification suggested in kit PowerPlex(tm)16 System (Promega).

5-Mitochondrial DNA study

The following methodology was used in the Mitochondrial DNA study:

PCR Amplification:

PCR Amplification of the human mtDNA regions HVI and HVII, using specified primers and amplification conditions in accordance with Vigilant et al., 1989 [4] e Wilson et al., 1995 [5], Alvarez-lglesias et al., [6].

[4] Vigilant L, Pennington R. Harpending 11, Kocher TD, Wilson AC. 1989. "Mitochondrial DNA Sequences in Single Hairs from a Southern African Population". Proc Natl Acad Sci, USA, 1989, Vol. 86: 9350 - 9354.
[5] Wilson MR, DiZinno JA, Polanskey D, Replogle J. Budowle B. 1995. "Validation of Mitochondrial DNA Sequencing for Forensic Casework Analysis". Int J Legal Med. 1995,108: 68 - 74.
[6] Alvarez-Iglesias V, Jaime JC, Carracedo A, Salas A.2007. "Coding region mitochondrial DNA SNPs:Targeting East Asian and Native American haplogroups" Forensic Sci. Int. Genetics, 2007, 1 :44-55.

Sequenciation:
Direct sequenciation of the HVI e HVll segments by capilar electrophoresis in "ABI PRISM 3100 ou 3130 Genetic Analyser" (Applied Biosystems) automated sequenciator with fluorescent detection using the following kits:

- "BigDye(R) Terminator vl.1 Cycle Sequencing" (Applied Biosystems)
- "BigDye(R) Terminator v1.3 Cycle Sequencing" (Applied Biosystems)
- "dRhodamine Terminator Cycle Sequencing Ready Reaction" (Applied Biosystems)

The programme "SeqScape(R) Software Version 2.0" (Applied Biosystems) was used for comparison of the sequences obtained from the analysed samples with the CRS (Cambridge Reference Sequence).



1- Morphology analysis of the hairs

All hairs received for analysis were subjected to macro- and microscopic characterisation, with the majority of those having the root being in a telogenic phase.
[Ref: https://www.promega.com/geneticidproc/e ... c/ab20.pdf ]

Relating to Apartment n° 5-A

- Envelope N° 1 - "recovered from the floor at the entrance to the children's bedroom" 25 hairs with root; 4 hairs only stem; 3 hairs non-human.

- Envelope N° 2 - "recovered from the floor next to the bed from which the child disappeared" 3 hairs with root; 25 hairs only stem.

- Envelope N° 3 - "recovered from the top of the bed from which the child disappeared" 2 hairs with root; 2 hairs only stem.

- Envelope N° 4 - " recovered from the floor next to the bed that was next to the window in the children's bedroom" ll hairs with root; 4 hairs only stem.

- Envelope n° 6 - "recovered from the floor of the lounge" 26 hairs with root; 4 hairs only stem; l hair non-human.

- Envelope n° 7 - "recovered from the entrance hall at the front door of the apartment" 44 hairs with root; 8 hairs only stem; 6 hairs non-human.

Relating to the Volkswagen 44-77-KD

- Envelope n° 1 - "recovered from the front-right seat" 13 hairs with root; 1 hair root only; 1 hair non-human.

- Envelope n° 2 - "recovered from the front-left seat" 2 hairs with root.

- Envelope n° 3 - "recovered from the floor in front of the front seats" 9 hairs with root; 2 hairs only stem; 1 hair non-human.

- Envelope N° 4 - "recovered from the floor in front of the rear seat" 1 hair with root; 6 hairs only stem.

- Envelope N° 5 - "recovered from the rear seat" 4 hairs only stem.

- Envelope N° 6 - "recovered from the rear part of the vehicle" 1 hair with root; 4 hairs only stem.

- Envelope N° 7 - "recovered from the internal covering of the roof" 3 hairs with root; 1 hair only stem.

Relating to the Residencia Casa Liliana - Praia da Luz-Lagos:

- Envelope N° 1 - "recovered from the bed in the bedroom of of the suspect" 36 hairs with root; 4 hairs only stem.

2-Nature of the sample

Acid Phosphatase Test to detect semen on the small spot on the cloth fragment in envelope n° 5 recovered from the bedspread of the bed next to the window of the children's bedroom: Weakly positive.

3 - Nuclear DNA study

Autosomic STRs:

- were obtained from: hair root CEnv 7-51 recovered in the apartment (entrance hall-Env.7), hair root PVT07 recovered in the vehicle (floor in front of rear seat Env.4), hair root C742-05 recovered in the vehicle (floor in front of the front seat- Env. 3) and the small spot on the piece of cloth (recovered from the bedspread of the bed next to the window in the children's bedroom Env.5).

- were obtained from swabs:

Matthew David Oldfield, Jane Michelle Tanner, Gerald Patrick Mccann, Kate Marie Healey, Dianne Webster, Rachael Mariamma Jean Mampilly, Russell James O'Brien, Fiona Payne, David Anthony Payne, Silvia Maria Correia Batista e Robert James Queriol Eveleigh Murat.

4 - Mitochondrial DNA study

- Mitochondrial DNA was obtained from 194 vestiges - 1 spot and 193 hairs (roots and/or stems) of the 246 analysed. It was not possible to obtain results from 52 hairs, 31 of which were recovered in the apartment.


Mitochondrial DNA profiles of the vestiges

* Bl and Dl have a different sequence from B and D.



1st- The macro- and microscopic analysis of 257 hairs revealed 245 human and 12 non-humano.

2nd- With respect to autosomic STRs the genetic profiles of the following analysed samples matched with the reference sample indicated::

- hair root CEnv 7-51 recovered in the apartment (entrance hall-Env.7) matched with Gerald Mccann, father of the victim.

- hair root PVT07 recovered in the vehicle (floor in front of rear seat Env.4) match with Robert James Queriol Eveleigh Murat.

3rd- With respect to autosomic STRs the female genetic profile identified from hair root C742-05 recovered in the vehicle(floor in front of the front seat- Env 3) did not match any profile from the reference samples.

4th- With respect to autosomic STRs the male genetic profile identified from the spot on the cloth fragment (bedspread of the bed next to window in children's bedroom- Env 5) did not match any profile from the reference samples.

5th- In the samples from apartment 5-A, several mitochondrial DNA profiles were found:

- Profile identified by letter "C", present in 53 samples, was identical to that of Kate Healy, mother of the victim, meaning those samples were from her or from someone having the same maternal bloodline.

- Profile identified by letter "N", present in 24 samples, was identical to that of Gerald Mccann, father of the victim, meaning those samples were from him or from someone having the same maternal bloodline.

- Profile identified by letter "G", present in 1 samples, was identical to that of Matthew David Oldfield, meaning that sample was from him or from someone having the same maternal bloodline.

- Profile identified by letter "I", present in 1 samples, was identical to that of David Anthony Payne, meaning that sample was from him or from someone having the same maternal bloodline.

- Profile identified by letter "O", present in 2 samples, was identical to that of Russell James O'Brien, meaning those samples were from him or from someone having the same maternal bloodline.

- Profile identified by letter "L", present in the spot on the bedspread of the bed next to the window and in seven hairs, meaning that all these samples came from the same person or from someone having the same maternal bloodline, did not match any of the reference samples.

- Profiles identified by letters "B", "D", "F", "J" and "Q" are different from the above, and from each other, and are distinct from reference samples.

6th- In the samples analysed from Residencia Liliana and the vehicle, several mitochondrial DNA profiles were found:

- Profile identified by letter "M", present in 48 samples (35 from Residencia Liliana and 13 from the vehicle), was identical to that of Robert James Queriol Eveleigh Murat meaning those samples were from him or from someone having the same maternal bloodline.

- Profile identified by letter "K", present in 14 samples (1 from Residencia Liliana and 13 from the vehicle), meaning that all these samples came from the same person or from someone having the same maternal bloodline, did not match any of the reference samples.

- One of the 13 samples from the vehicle in letter "K" - hair root C742-05 (floor in front of the front seat - Env 3) showed a female DNA genetic profile.

7th- The remaining 28 samples analysed, of which 17 were recovered from the apartment, showed mitochondrial DNA from different from each other, and distinct from those above.

8th- Of the 245 hairs analysed, no results were obtained from 52.

National Institute of Legal Medicine, 9 July 2007.

[Titles, names and signatures of signatories to the report]

Vol IX

Page 2419

Reply from the Forensics Institute (INML) to Goncalo Amaral

22 – 08 - 2007

Reply to Queries

- We inform you that none of the samples received by this institute were designated as supposedly belonging to the missing girl and we therefore, cannot reply to this query.


- Samples were studied – hair and a piece of cloth – nuclear DNA profiles only being obtained from 4 samples, which upon comparison with the DNA profiles of Kate and Gerry, could not belong to the girl.

- The samples were then studied using mitochondrial DNA analysis, the same was done for the other samples, giving the results in accordance with our report of 9th July Nº 2007/000226 LX-BC.

- As requested in point 5, it was determined that the profile obtained by the British lab could belong to a son/daughter of the McCanns.


- The comparison of the profiles obtained in autossomic STR from Kate and Gerry McCann with the profile obtained was carried out.


Signed


(Screen shot of profiles pending)

Processos Vol XIII

Pages 3578 to 3590 (in English, PJ translation I presume)
Same text also in Vol XIII pages 3940 to 3948.

(Note: There is no date for this correspondence, neither is there a reference as to whom it is addressed).


a. List of samples collected from 5A and examined and report of events and evidence collected

On May 3rd 2007, under unknown circumstances, a child – Madeleine Beth McCann – went missing from the apartment she was sharing with her parents, brother and sister, at the resort “Ocean Club”, located in Praia da Luz, Lagos.

At a first stage of the investigation, confronted with the disappearance, while considering that the child could have walked out of the apartment, on her own, and could have got lost in the area, the abduction theory was the one which achieved more consistency, partly due to the parents suspicions. After police intervention, the searches already started furthered and progressively enlarged to gradually farther distant area, with the involvement of search dog teams and numerous human resources.

During that stage of the investigation, the apartment was searched by members of the Crime Scene Team from the Laboratorio de Policia Cientifica [Police Forensic Science Laboratory] of the Portuguese Criminal Police, and samples were collected. Those samples, namely hair and a sperm stain, were sent to the National Institute of Legal Medicine (INML)to be analysed taking into account the DNA profile of the missing child-that had been provided-, as well as one of her parents and the ones of friends who belonged to a common group, and who went to the above mentioned tourist resort following previous arrangements among them.

Following the forensic tests carried out at INML, some DNA were obtained which linked profiles of some people within the group of friends, to the profiles of the child’s parents and even to the profiles of other people at the site. Until this moment, unidentified mitochondrial DNA profiles were also obtained. On the other hand, it was possible to define the autosomic STR profile of the sperm stain detected on the bedspread of one of the beds in the bedroom from where the child went missing. It has been already checked that there is no matching between the obtained genetic profile and the profile of the various samples of reference, which include the ones of the parents, friends, and others. Therefore, that DNA profile is now being forwarded to you, so that it can be inserted in your database, with the request that you check if it matches any of the genetic profiles from 3 British citizens who had stayed in the apartment in question, before the McCanns started to use that apartment on May 3rd, 2007.

Thus, and considering the submitted samples, which preliminary report has already been forwarded to us, and in order to provide a better outline and classification, we hereby indicate the data and the circumstances of that sample collection. Furthermore, we also enclose some queries which answers we would like you to include in your final report. We are looking forward to receiving it and we appreciate it could be submitted to us as soon as possible.

b) Samples collected in the living room of the apartment 5A, block A, Ocean Club, tourist resort – Praia da Luz, Lagos, on August 1st, 2007, following the examination carried out by a British specialist dog team. Delivery note no. 286/2007 –CR/L):

1. Pieces of tile number 1.
2. Pieces of tile number 2.
3. Pieces of tile number 3.
4. Pieces of tile number 4.
5. Pieces of tile identified next to tile number 1.
6. Pieces of skirting board number 1.
7. Pieces of skirting board number 2.
8. Pieces of skirting board identified between skirting boards 2 and 3.
9. Pieces of skirting board number 3.
10. Pieces of skirting board number 4.
11. Pieces of the skirting board identified next to skirting board 1.
12. Pieces of the skirting board identifies next to skirting board 4.
13. Residues/small fragments from the area of the collected skirting boards.
14. Residues/small fragments from the are of the collected tiles.
15. Existing grout between the tiles and the upper area of the collected tiles.
16. Existing grout around tile 1.
17. Existing grout around tile 2.
18. Existing ground around tile 3.
19. Existing grout around tile 4.
20. Residues of tiles, cement-glue and existing grout in the area from where the tiles were collected.
21. Fragments of bushes.

Mouth swabs collected from the below mentioned crime scene examiners are also added hereby. Those examiners carried out the sample collections aimed at identifying possible contamination.
22. Two mouth swabs from Fernando Jose Silva Viegas.
23. Two mouth swabs from Lino Manuel Lima Henriques.
24. Two mouth swabs from Bruno Jorge P Ossidonio Mendes Antunes.

b) Samples collected in the living room of the apartment 5A, block A, Ocean Club, tourist resort, Praia da Luz, Lagos, on August 3rd, 2007, following the second examination carried out by a British specialist dog team (Delivery note no. 286B/2007-CR/L):

1. A piece of fabric from white curtain next to a window in the living room.
C) Samples collected in the living room of the apartment 5A, block A, Ocean Club tourist resort, Praia Da Luz, Lagos, on August 4th , 2007, following the second examination carried out by a British specialist dog team (Delivery note no. 286A/2007-CR/L):
1A. Stain on the floor collected using a dry swab.
1B. Stain on the floor collected using a swab, wet with distilled water.
2A. Stain on the floor collected using a dry swab.
2B. Stain on the floor collected using a swab, wet with distilled water.
3A. Stain on the floor collected suing a dry swab.
3B. Stain on the floor collected using a swab, wet with distilled water.
4A. Stain on the floor collected using a dry swab.
4B. Stain on the floor collected using a swab, wet with distilled water.
5A. Stain on the wall using a dry swab.
5B. Stain on the wall collected using a swab wet with distilled water.
6A. Stain on the floor collected using a dry swab.
6B. Stain on the wall collected using a swab wet with distilled water.
7A. Stain on the floor collected using a dry swab.
7B. Stain on the wall collected using a swab wet with distilled water.
8A. Stain on the floor collected using a dry swab.
8B. Stain on the wall collected using a swab wet with distilled water.
9A. Stain on the floor collected using a dry swab.
9B. Stain on the wall collected using a swab wet with distilled water.
10A. Stain on the floor collected using a dry swab.
10B. Stain on the wall collected using a swab wet with distilled water.
11A. Stain on the floor collected using a dry swab.
11B. Stain on the wall collected using a swab wet with distilled water.
12A. Stain on the floor collected using a dry swab.
12B. Stain on the wall collected using a swab wet with distilled water.
13A. Stain on the floor collected using a dry swab.
13B. Stain on the wall collected using a swab wet with distilled water.
14A. Stain in the rear of a sofa collected using a dry swab.
14B. Stain on the wall behind a sofa collected using a swab wet with distilled water.
15A. Stain in the rear of a sofa collected using a dry swab.
16. Two curtains made of blue fabric.
16B. One curtain made of white fabric, and a blue coloured tieback.

d) Samples collected in the metallic grey Renault Scenic vehicle, number plate 59-Da-27, on August 6th, 2007, following the second examination carried out by a British specialist dog team (Delivery note no. 286C/2007-CR/L):

1A. Head hair collected from the driver’s seat.
2A. Head hair collected to the right of passenger’s seat.
2B. Head hair collected from the floor next to the right front passenger’s seat.
3. Head hair collected between the front seats.
4A. Head hair collected from the left area of the back seat.
5A. Head hair collected from the middle area of the back seat.
6A. Head hair collected from the right area of the back seat.
7A. Head hair collected from the left seat of the luggage area.
8A. Head hair collected from the right seat of the luggage area.
9. Head hair collected from the vehicle luggage area.

1D. Nail fragment collected in the floor in front of the driver’s seat.
2E. Nail fragment collected in the floor in front of the driver’s seat.

1B. Fibres and possible head hair from the back of the driver’s seat.
1C. Fibres and possible head hair from the bottom of the driver’s seat.
2C. Fibres and possible head hair from the back of the right front passenger’s seat.
2D. Fibres and possible head hair from the bottom of the right front passenger’s seat.
4B. Fibres and possible head hair from the back in the left area of the back seat.
4C. Fibres and possible head hair from the bottom in the left area of the back seat.
5B. Fibres and possible head hair from the back in the middle area of the back seat.
5C. Fibres and possible head hair from the bottom in the middle area of the back seat.
6B. Fibres and possible head hair from the back in the right area of the back seat.
6C. Fibres and possible head hair from the bottom in the right area of the back seat.
7B. Fibres and possible head hair from the back of the left seat in the vehicle luggage area.
7C. Fibres and possible head hair from the bottom in the left area of the back seat.
8B. Fibres and possible head hair from the back of the right seat in the vehicle luggage area.
8C. Fibres and possible head hair from the bottom of the right seat in the vehicle luggage area.
11. Fibres and possible head hair in the rear shelf/luggage cover.
13. Pattern sample of the fabric covering the vehicle seats.


Material identified during the inspection carried out by the specialist dog team:

10. Parts of the vehicle luggage area.
12. Vehicle ignition key.


We also enclose mouth swabs and pattern samples taken from the clothes worn by the Portuguese fingerprint expert, Pedro Miguel Filipe Goncalo Vilhena, who drove the vehicle under examination, with a view to allowing for possible contamination screening.

14. Two mouth swabs from Pedro Miguel Filipe Goncalo Vilhena.
15. Pattern samples taken from the clothes worn by Pedro Miguel Filipe Goncalo Vilhena.

Queries:
As for trace evidence in the Delivery Note no. 286/207-CR/L is regarded, we request that:
• the biological traces concerning items 1 to 20 be researched, and their nature and respective DNA profiles determined (if possible).
• the blood traces concerning item 21 be researched, and their respective DNA profiles determined.

As far as the trace evidence in the Delivery Note no. 286B/2007 – R/L is concerned, we hereby request that any biological evidence be researched, and its nature and respective DNA profiles determined (if possible).

As for the trace evidence in the Delivery note no. 286A/2007-CR/L, we request that
• The nature of biological trace evidence concerning items 1A to 15B be determined (if possible), as well as its respective DNA profiles.
• The biological trace evidence regarding items 16 and 16B be researched, and its nature and respective DNA profiles determined (if possible).

As regards the trace evidence in the Delivery Note no. 286C/2007-CR/L, we hereby request you to:
• Determine whether head hair samples 1A, 2A, 2B, 3, 4A, 5A, 6A, 7A, 8A, and 9, as well as occasional head hairs contained in items 1B, 1C, 2C, 2D, 4B, 4C, 5B, 5C, 6B, 6C, 7B, 7C, 8B, 8C, and 11 originate from ante mortem or post-mortem deposition;
• Determine its respective DNA profiles, as well; and in case some of these samples are identified as belonging to the missing child, provide a toxicological research for any medicinal substances present therein;
• Determine the respective DNA profiles regarding items 1D and 2E;
• Research biological trace evidence concerning items 10 and 12, and determine (if possible) its biological nature and respective DNA profiles;
• Compare fibres present in items 1B, 1C, @C, 2C, 4B, 4C, 5B, 5C, 6B, 6C, 7B, 7C, 8B, 8C, and 11 with the pyjamas items in the Delivery Note no. 397/2007-CR/L. taking into account the standard samples from the fabric covering the vehicle seats (items 13) and the vehicle driver’s clothing (items 15).

We further request the DNA profile comparison concerning the English citizens that stayed in the apartment throughout 2007, where the above-referred sperm stain was found.
-FAWKES, SIMON ANDREW
-DAMBROSIO, CARLO
-GORDON, PAUL ANTHONY

With the DNA profile

D3S1358 15-18 CSFIPO 10-11
HUMTHOI 9.3 Penta D 9
D21511 29-32.2 VWA 18
D18551 17-18 D8S1179 12-13
Penta E 7-8 TPOX 11
D5S818 12-13 Fibra 20-24
D13S317 12-14 D2SI338 18-25
D75820 10-12 D19S433 13-15
D16S539 11 Amelogemine XY

Obtained by the INML, IP from a bedspread of a bed located next to the window of the children’s bedroom in Apartment 5A, block A, in the Ocean Club, tourist resort, Praia da Luz, Lagos, with the results obtained by this laboratory.

At last, we request a reply as regards the previous queries concerning the collected mouth swabs from NEIL BERRY and RAJINDER RAJ SING BALU, as well as their DNA profiles comparisons with the results obtained in that laboratory, and also the comparison with the mitochondrial DNA profiles already submitted.

Best Regards,
Criminal Investigation Superior Coordinator

Paulo Rebelo

*****Page 3590*****
Further to our previous message regarding dates and circumstances concerning the collection of the evidence to be examined in your laboratory, along with the enclosed queries, we further inform you that a pair of pyjamas will be later handed over (delivery note 397/2007-CR/L), similar to those Madeleine Beth McCann was wearing when she went missing.

Best regards,
Criminal Investigation Superior Coordinator

Paulo Rebelo

Processos Vol IV

Page 836


To: The Coordinator of the Criminal Investigation


Date: 2007/05/09

Subject: Laboratory Analysis of the samples gathered with regard to the disappearance of the child Madeleine McCann.

Dear Sir,

In accordance with the request made in your fax nº 388 of 8th May 2007, the samples collected by officers from the Local Crime Scene of the LPC concerning the examination of Apartment 5ª of the OC in P da L, were delivered to the Genetics and Biological Forensics Service of the INML – Lisbon delegation, in order for the respective laboratory analyses to be carried out,.

According to the information provided by Dra Ribeiro, from the previously mentioned Institute, the analysis of the samples will begin from today, however the urgent delivery of mouth swabs, containing the biological samples of each of the parents and the siblings is necessary as well as those of the tourist group who were on holiday with them.

I hereby request the urgent delivery of the mouth swabs referred to as well as a letter requesting the implementation of the laboratory analyses, in order to be able to carry them out, this letter should be sent to the Genetics and Forensic Biology Service of the INML, Fax****.

With compliments.


Director of the LPC.

Processos Vol IV


Page 904

Statement


I, Gerald Patrick McCann...................................hereby declare my authorisation, for the purposes of DNA examination, that a mouth swab be taken from me at the Portimao DIC.

Portimao, 10th May 2007


Signed

G. McCann

Processos Vol VII

Page 1804

I, Kate Marie Healy...................................hereby declare my authorisation, for the purposes of DNA examination, that a mouth swab be taken from me at the Portimao DIC.

Portimao, 10th May 2007


Signed

K. Healy

Processos Vol VII


Page 1827

From: D Corte, INML
To: Luis Neves


Date: 19th June 2007



Dear Dr Luis,

I enclose the data from the analysis of all the samples. We will repeat some that did not give results.


With compliments

F. Corte Real


Please can someone provide a screen shot of the following page:


Processos Vol VII
Page 1828
Profiles of mitochondrial DNA from the samples sent

Table of Contents : Processo Vol 8...Pages 2204 to 2210—"Delivery documents of all vestiges collected (English) sent to Birmingham 7th August. 2007

Processo Vol 8...Pages 2204-2210

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Page 2204 :

(Delivery guide of recovered evidences at the crime scene)

In order to carry out the determined by the 4.a Brigada of Departamento of Investigação Criminal of Polícia Judiciária, concerning the process number 201/07.OGALGS, on the 1st August, 2007, between 20:00h and 01:30h of the 2nd August, 2007, were recovered the following evidences in the living room of the apartment 5A, Ocean Club Villas, Praia da Luz, Lagos, Portugal, where it is possible may have occurred a crime of homicide, by the Crime Scene Investigators Fernando José da Silva Viegas and Lino Manuel Lima Henriques:-

1- Tile pieces identified with number 1;
2- Tile pieces identified with number 2;
3- Tile pieces identified with number 3;
4- Tile pieces identified with number 4;
5- Tile pieces identified near number 1;
6- Pieces of skirting identified with number 1 ;
7- Pieces of skirting identified with number 2;
8- Pieces of skirting identified between number 2 and 3;
9- Pieces of skirting identified with number 3;
10- Pieces of skirting identified with number 4;
11 - Pieces of skirting identified nearby number 1 ;
12- Pieces of skirting identified nearby number 4;
13- Residue / small fragments of the skirting area;
14- Dust / small fragments of the tile area;
15- Cement glue between the tiles in the edge of the recovered area;
16- Cement glue between the tile identified with number 1;
17- Cement glue between the tile identified with number 2;
18- Cement glue between the tile identified with number 3;
19- Cement glue between the tile identified with number 4;
20- Leftovers of tiles and cement glue;


Page 2205 :

21- Fragments of bushes;
22- Two mouth swabs from CSI Fernando Jose Silva Viegas;
23-Two mouth swabs from CSI Lino Manuel Lima ~enriques;
24- Two mouth swabs from CSI Bruno Jorge Possidónio Mendes Antunes.

All these evidences were delivered to the Forensic Science Laboratory - Birmingham Laboratory, Priory House, Gooch Street North, Birmingham, B56QQ, on the 7th August, 2007.-

Birmingham, August, 7th, 2007

Who delivers - Illegible, (to me), handwritten Signature.

Who received - S NIBLETTS, FSS Birmingham ( plus handwritten Signature)

Dated 07 August 2007



Page 2206 :

(Delivery guide of recovered evidences at the crime scene)

In order to carry out the determined by the 4.a Brigada of Departamento of Investigação Criminal of Policia Judiciária, concerning the process number 201/07.OGALGS, between 15:00h of 4th August, 2007, and 06:30h of 5th August, 2007, were recovered the following evidences in the living room of the apartment 5A, Ocean Club Villas, Praia da Luz, Lagos, Portugal, where it is possible may have occurred a crime of homicide, by the Crime Scene Investigators Fernando José da Silva Viegas and Bruno Jorge Possidónio Mendes Antunes:-

I A - Stain on the floor recovered with a Dry swab;
1 B - Stain on the floor recovered with swab with distilled water;
2A - Stain on the floor recovered with a Dry swab;
2B - Stain on the floor recovered with swab with distilled water:
3A - Stain on the floor recovered with a Dry swab;
3B - Stain on the floor recovered with swab with distilled water;
4A - Stain on the wall recovered with a Dry swab;
4B - Stain on the wall recovered with swab with distilled water;
5A - Stain on the wall recovered with a Dry swab;
5B - Stain on the wall recovered with swab with distilled water;
6A - Stain on the wall recovered with a Dry swab;
6B - Stain on the wall recovered with swab with distilled water;
7A - Stain on the wall recovered with a Dry swab;
7B - Stain on the wall recovered with swab with distilled water;
8A - Stain on the wall recovered with a Dry swab;
8B - Stain on the wall recovered with swab with distilled water;
9A - Stain on the wall recovered with a Dry swab;
9B - Stain on the wall recovered with swab with distilled water;
10A - Stain on the wall recovered with a Dry swab;
10B - Stain on the wall recovered with swab with distilled water;



Page 2207 :

11IA - Stain on the wall recovered with a Dry swab;
11 B - Stain on the wall recovered with swab with distilled water;
12A - Stain on the wall recovered with a Dry swab;
12B - Stain on the wall recovered with swab with distilled water;
13A - Stain on the wall recovered with a Dry swab;
13B - Stain on the wall recovered with swab with distilled water;
14A - Stain on the back of the sofa recovered with a Dry swab;
14B - Stain on the back of the sofa recovered with swab with distilled water;
15A - Stain on the back of the sofa recovered with a Dry swab;
15B - Stain on the back of the sofa recovered with swab with distilled water;
16 - Blue curtain;
16B - White curtain behind blue curtain and armband.

All these evidences were delivered to the Forensic Science Laboratory - Birmingham Laboratory, Priory House, Gooch Street North, Birmingham, B56QQ, on the 7th August, 2007.-

Birmingham, 7th August, 2007

Who delivers - Illegible, (to me), handwritten Signature.

Who received - S NIBLETTS, FSS Birmingham ( plus handwritten Signature)

Dated 07 August 2007



Page 2208 :

(Delivery guide of recovered evidences at the crime scene)

In order to carry out the determined by the 4.a Brigada of Departamento of Investigação Criminal of Policia Judiciária, concerning the process number 201/07.OGALGS, on the 3rd August, 2007, were recovered the following evidence in the living room of the apartment 5A, Ocean Club Villas, Praia da Luz, Lagos, Portugal, where it is possible may have occurred a crime of homicide, by the Fingerprint Expert Teresa Borges:-

1 - Piece of curtain.

This evidence was delivered to the Forensic Science Laboratory - Birmingham Laboratory, Priory House, Gooch Street North, Birmingham, B56QQ, on the 7th August, 2007.-

Birmingham, 7th August, 2007

Who delivers - Illegible, (to me), handwritten Signature.

Who received - S NIBLETTS, FSS Birmingham ( plus handwritten Signature)

Dated 07 August 2007



Page 2209 :

(Delivery guide of recovered evidences at the crime scene)

In order to carry out the determined by the 4.a Brigada of Departamento of Investigação Criminal of Policia Judiciária, conceming the process number 201/07.OGALGS, between 21:30h of 6th August, 2007, and 06:00h of 7th August, 2007, were recovered the following evidences in a vehide Renault Scenic, colour grey, licence plate 59-DA-27 by the Crime Scene Investigators Femando José da Silva Viegas and Lino Manuel Lima Henriques:-

IA - Hair in the driver's place;
1 B - Fibres and possibly hairs on vertical part of the seat of left side front;
1C - Fibres and possibly hairs on horizontal part of the seat of left side front;
1 D - Nail on the driver's floor;
2A - Hair in the right side front seat;
2B - Hair in the right side front floor;
2C - Fibres and possibly hairs on vertical part of the seat of right side front;
2D - Fibres and possibly hairs on horizontal part of the seat of right side fmnt;
2E - Nail in the right side front floor;
3 - Hair between the front seats;
4A - Hair in the back seat left;
4B - Fibres and possibly hairs on vertical part of the back seat left;
4C - Fibres and possibly hairs on horizontal part of the back seat left;
5A - Hair in the back seat centre;
5B - Fibres and possibly hairs on vertical part of the back seat centre;
5C - Fibers and possibly hairs on horizontal part of the back seat centre;
6A - Hair in the back seat right;
6B - Fibres and possibly hairs on vertical part of the back seat right;
6C - Fibres and possibly hairs on horizontal part of the back seat right;
7A - Hair in the left seat on the luggage compartment;



Page 2210 :

7B - Fibres and possibly hairs on vertical part of the left seat on the luggage compartment;
7C - Fibres and possibly hairs on horizontal part of the left seat on the luggage compartment;
8A - Hair in the right seat on the luggage compartment;
8B - Fibres and possibly hairs on vertical part of the right seat on the luggage compartment;
8C - Fibers and possibly hairs on horizontal part of the right seat on the luggage compartment;
9 - Hair in luggage compartment;
10 - Top of the luggage compartment;
11 - Fibres and possibly hairs on the boot mat;
12 - Car key;
13 - Control samples of seat tissues;
14 - Two mouth swabs from Fingerprint Expert Pedro Miguel Filipe Gonçalo Vilhena;
15 - Fibres recovered from Fingerprint Expert Pedro Miguel Filipe Gonçalo Vilhena;

All these evidences were delivered in the Forensic Science Laboratory - Birmingham Laboratory, Priory House, Gooch Street North, Birmingham, B56QQ, on the 7th August, 2007.-

Birmingham, August, 7.th, 2007

Who delivers - Illegible, (to me), handwritten Signature.

Who received - S NIBLETTS, FSS Birmingham ( plus handwritten Signature)

Dated 07 August 2007

Processos Vol VII
Page 1828
Profiles of mitochondrial DNA from the samples sent

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